Gelatin Hydrolysis Test | Principle, Uses, Media, Procedure and Result

Gelatin Hydrolysis Test

This test microorganisms used to measure the ability to synthesize enzymes which hydrolyze gelatin gelatinase-. Bacteria which are used in identification. Gelatin, which is a substance derived from collagen protein hydrolyzation character. Because large molecule bacteria can not pass through cell walls. Therefore, smaller molecules is catalyzed; gelatinase- which makes it extra cellular enzyme. Catabolism of proteins by Gelatinase is twofold.

The gelatin is partially hydrolyzed found at high temperature for a long time and loses its cure rates.

Technical Test Procedure; 

• The other taken with a needle to extract from the bacterial colonies to be examined is done planting gelatinous medium. 
• The tubes are incubated at 37 ° C up to 15 days. 
• After this time the refrigerator temperature with controls allowed for 1-2 hours. 
• Care is taken whether to melt. Where the hydrolysed gelatin is then removed from the refrigerator, as a liquid medium and appeared to solidify the gelatin (positive gelatin hydrolyzing). in vitro liquid medium in the negative case gelatine is solidified.

Positive: Partial or complete liquefaction of the inoculated tube at 4°C. The control tube remain solidified even after exposure to cold temperature.Examples: Aeromonas hydrophila, Bacillus subtilis

Negative: At the end of the refrigeration, the control tube and the test tube both remain completely solidified.
Examples: Micrococcus roseus, Escherichia coli

Quality Control

Positive: Pseudomonas aeruginosa ATCC 10145

Negative: Escherichia coli ATCC 25922

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