Uses
Şubat 27, 2019
Gelatin Hydrolysis Test | Principle, Uses, Media, Procedure and Result
Gelatin Hydrolysis Test
This test microorganisms used to measure the ability to synthesize enzymes which hydrolyze gelatin gelatinase-. Bacteria which are used in identification. Gelatin, which is a substance derived from collagen protein hydrolyzation character. Because large molecule bacteria can not pass through cell walls. Therefore, smaller molecules is catalyzed; gelatinase- which makes it extra cellular enzyme. Catabolism of proteins by Gelatinase is twofold.
The gelatin is partially hydrolyzed found at high temperature for a long time and loses its cure rates.
Technical Test Procedure;
- The other taken with a needle to extract from the bacterial colonies to be examined is done planting gelatinous medium.
- The tubes are incubated at 37 ° C up to 15 days.
- After this time the refrigerator temperature with controls allowed for 1-2 hours.
- Care is taken whether to melt. Where the hydrolysed gelatin is then removed from the refrigerator, as a liquid medium and appeared to solidify the gelatin (positive gelatin hydrolyzing). in vitro liquid medium in the negative case gelatine is solidified.
Positive: Partial or complete liquefaction of the inoculated tube at 4°C. The control tube remain solidified even after exposure to cold temperature.Examples: Aeromonas hydrophila, Bacillus subtilis
Negative: At the end of the refrigeration, the control tube and the test tube both remain completely solidified.
Examples: Micrococcus roseus, Escherichia coli
Examples: Micrococcus roseus, Escherichia coli
Quality Control
Positive: Pseudomonas aeruginosa ATCC 10145
Negative: Escherichia coli ATCC 25922
INSTAGRAM
