mikrobiyoloji
Mart 04, 2019
HBsAg Test | What Does It Mean Positive or Negative Results | Cassette Test
HBsAg Test
HBsAg is a virus, the most common cause of acute hepatitis. As with all viruses forming hepatitis, hepatitis B virus (HBV) is the most serious form of the disease. Hepatitis B is an infectious disease caused by the hepatitis B virus.
Sitting of hepatitis B virus between 1 to 6 months. Upon determination of the HBsAg for at least 6 months in the serum of chronic HBV infection it occurs. Hepatitis B, mild and asymptomatic infection to more severe liver disease and cirrhosis to hepatocellular carcinoma meantime (liver cancer) can cause a number ranging table.
Sitting of hepatitis B virus between 1 to 6 months. Upon determination of the HBsAg for at least 6 months in the serum of chronic HBV infection it occurs. Hepatitis B, mild and asymptomatic infection to more severe liver disease and cirrhosis to hepatocellular carcinoma meantime (liver cancer) can cause a number ranging table.
Hepatitis B virus, placing their genetic material into the liver cell, provide reproductive mechanism of proliferation of these cells by routine. The human body immune system, containing the genetic material of the virus to attack his own liver cells into action. So the virus damages the liver indirectly. incessant attacks of the immune system, resulting in damage and the death of liver cells.
Hepatitis B virus; blood products, dental treatment, sexual intercourse, sharing needles in intravenous drug use, tattoos made with contaminated instruments, acupuncture and ear during the piercing process and can be transmitted from mother to baby at birth.
Today HBsAg test, as in the microbiology lab and commercial produced by different companies otoanalyser "tape test" is conducted NSAIDs.
This test sample in 0.5ng / ml HBsAg virus can detect the remaining amount.
Today HBsAg test, as in the microbiology lab and commercial produced by different companies otoanalyser "tape test" is conducted NSAIDs.
This test sample in 0.5ng / ml HBsAg virus can detect the remaining amount.
Goal
Based immunochromatographic of hepatitis B surface antigen in human serum or plasma is determined qualitatively.
Wherewithal
- Vial (made of heat-resistant plastic material usually screw cap sample storage tube)
- Centrifuge
- HBsAg test cassette
- Disposable plastic pipettes
- Fresh human serum or plasma sample
- The test cassette is brought to room temperature.
- removed from the test tray, the tea bag is placed on a flat surface.
- Sample 2-3 drops hole plasma / serum added dropwise. Air bubbles should be avoided.
Evaluation Test
Test results are read after 10-20 minutes.
Formed after 20 minutes is not an attribute of any line in terms of diagnosis and should not be considered any results that occurred after 20 minutes.
Results window only "C" in the section on the remaining one-line letter indicates a negative result.
And the result window "C" and "T" in the section lines remaining on the letters indicates a positive result.
At the end of the period to be expected no invisible line in the result window, the result is invalid. Experiments should be repeated with a new test.
Considerations Rules of HBsAg Cassette Test
Please read the instructions carefully before using the User.
Never freeze the test cassette. Bring to room temperature before you use the test refrigerated products. Experiments at room temperature (15-30 ° C) should be performed.
Do not use expired test expiration.
Whenever you remove from the bag immediately use the test cassette.
Do not use the test cassette torn bag.
Use disposable gloves during the test
Material mouth, face, eye care and avoid contact with the exposed wound.
Do not eat or smoke during the experiment.
Hemolytic, lipemic or your use of the samples containing bacteria.
INSTAGRAM
