Rose Bengal Test
Brucella abortus is to diagnose
Wherewithal
- Antigen (Rose bengal dye special technique stained with buffered saline to Brucella abortus bacteria are the standard suspension.)
- 1.5 cm diameter shallow pits with plates
- Patient serum
- Toothpick
Technical
➤ One of the plate wells, 0.03 ml of patient serum is placed.
➤ 0.03 ml of patient's serum on antigen mixed thoroughly put
➤ a toothpick spread over an area of 1.5 cm diameter with good stirring.
➤ Obtained or converted by circular movement on a rotator for 4 minutes.
➤ While the end results are read.
➤ One of the plate wells, 0.03 ml of patient serum is placed.
➤ 0.03 ml of patient's serum on antigen mixed thoroughly put
➤ a toothpick spread over an area of 1.5 cm diameter with good stirring.
➤ Obtained or converted by circular movement on a rotator for 4 minutes.
➤ While the end results are read.
Evaluation Test
➤ Resulting coarser precipitates (if agglutination) occurs; positive
➤ If the fine-particle precipitate was formed; suspicious
➤ Homogenous image is formed; It considered negative.
➤ Resulting coarser precipitates (if agglutination) occurs; positive
➤ If the fine-particle precipitate was formed; suspicious
➤ Homogenous image is formed; It considered negative.
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