Microbiology Laboratory Turkey

Mikrobiyoloji Ile Ilgili Tüm Konuların Kısa ve Öz Anlatımları. Microbiology Lab Information.

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MicroLab

23 Ekim 2018 Salı

Rose Bengal Test

Rose Bengal Test 

Brucella abortus is to diagnose

Wherewithal
  • Antigen (Rose bengal dye special technique stained with buffered saline to Brucella abortus bacteria are the standard suspension.) 
  • 1.5 cm diameter shallow pits with plates  
  • Patient serum 
  • Toothpick 

Technical  
➤ One of the plate wells, 0.03 ml of patient serum is placed. 
➤ 0.03 ml of patient's serum on antigen mixed thoroughly put 
➤ a toothpick spread over an area of 1.5 cm diameter with good stirring. 
➤ Obtained or converted by circular movement on a rotator for 4 minutes. 
➤ While the end results are read.


Evaluation Test  
➤ Resulting coarser precipitates (if agglutination) occurs; positive  
➤ If the fine-particle precipitate was formed; suspicious   
➤ Homogenous image is formed; It considered negative. 


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